PCR Genotyping Service

The Institute of Neuropathology has devoted a sizeable amount of work and resources to establishing an automated workflow - aided by a JANUS pipetting robot (PerkinElmer) - which allows to genotype animal biopsies efficiently and with a high throughput. Our efforts have been very successful, and this now enables us to offer our services to other researchers within the USZ.

Service:
1 PCR reaction = isolation of genomic DNA from an ear biopsy (transgenic or knockout animals) by specific primers, and analysis on a capillary electrophoresis system.

The PCR results (example), including images of the PCR reaction, are made available online on a password-protected platform.

Price list:
PCR genotyping service: 7 - 11 CHF/PCR

For each mouse line, a list of the applicable PCR reactions will be defined. For new PCR reactions, clients must supply the primer sequences along with a validated protocol.
http://www.iMice.uzh.ch/ Online platform for orders and results (registered clients) 

iMice Registration for new clients
 
SOP_iMice_Probenentnahme.pdf

LTK Modul 10 - Spezielle Applikations- und Blutentnahmetechniken bei der Maus
 
Contact and support:
- PCR results: Karina Arroyo, Karina.Arroyo@usz.ch
- Biopsy handling: Mirzet Delic, Mirzet.Delic@usz.ch
- Database, User-login: Simon Stephan Simon.Stephan@usz.ch, Norbert Wey, norbert.wey@usz.ch
- Software: Monika Bieri